Effect of aeration and agitation on growth rate of Thermus thermophilus in batch mode

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• 作者：Meltem U.  ; Demirtas¹ ; Arati  ; Kolhatkar² ; John J.  ; Kilbane II  ; ;   ; ² ;   ; ¹ ;   ; ^{john.kilbane@gastechnology.org}
• 关键词：Thermus ; Thermus thermophilus ; fermentation ; aeration ; growth rate ; cell yield
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：95
• 期：2
• 页码：113-117
• 全文大小：657 K

文摘

The growth kinetics of Thermus thermophilus HB27 was investigated in rich medium (Thermus medium) under batch cultivation at 65¡ãC in 3-l fermentors. The growth and oxygen consumption rates were highly dependent on the aeration and agitation rates. Volumetric mass transfer coefficient (K_La, h^?) and hence oxygen transfer rate (OTR, mol m ^? h^?) into the fermentation broth increased with increased aeration and/or agitation rates. A K_La and OTR of 175.4 h^? and 31.7 mol m^?h^? respectively, corresponding to 500 rpm agitation and2 vvm aeration with a mixture of air and oxygen, were required to avoid oxygen limitation. The maximum growth rate (¦Ì_max, h^?), doubling time (t_D, h), and dry cell weight determined for T. thermophilus HB27 growing under these conditions were 0.27 h^?, 2.67 h, and 3g/l respectively. This cell yield is higher than any previously published reports for growth of Thermus cultures, including studies that employed pressurized bioreactors.