Discrimination of Korean ginseng (Panax ginseng Meyer) cultivar Chunpoong and American ginseng (Panax quinquefolius) using the auxin repressed protein gene

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• 作者：Jong-Hak Kim² ; ^☆ ; Min-Kyeoung Kim¹ ; ^☆ ; Hongtao Wang² ; Hee-Nyeong Lee² ; Chi-Gyu Jin² ; Woo-Saeng Kwon² ; Deok-Chun Yang² ; ^{dcyang@khu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; ^{deokchunyang@yahoo.co.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：multiplex PCR ; Panax ginseng ; P. quinquefolius ; polymorphism
• 刊名：Journal of Ginseng Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：40
• 期：4
• 页码：395-399
• 全文大小：1592 K

文摘

Korean ginseng (Panax ginseng) is one of the most important medicinal plants in the Orient. Among nine cultivars of P. ginseng, Chunpoong commands a much greater market value and has been planted widely in Korea. Chunpoong has superior quality “Chunsam” (1^st grade ginseng) when made into red ginseng.

Methods

A rapid and reliable method for discriminating the Chunpoong cultivar was developed by exploiting a single nucleotide polymorphism (SNP) in the auxin repressed protein gene of nine Korean ginseng cultivars using specific primers.

Results

An SNP was detected between Chunpoong and other cultivars, and modified allele-specific primers were designed from this SNP site to specifically identify the Chunpoong cultivar and P. quinquefolius via multiplex polymerase chain reaction (PCR).

Conclusion

These results suggest that great impact to prevent authentication of precise Chunpoong and other cultivars using the auxin repressed protein gene. We therefore present an effective method for the authentication of the Chunpoong cultivar of P. ginseng and P. quinquefolius.