Total integrated slidable and valveless solid phase extraction-polymerase chain reaction-capillary electrophoresis microdevice for mini Y chromosome short tandem repeat genotyping
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- 作者：Yong Tae Kima ; Dohwan Leea ; Hyun Young Heoa ; Jeong Eun Simb ; Kwang Man Woob ; Do Hyun Kima ; Sung Gap Ima ; Tae Seok Seoa ; seots@kaist.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Integrated microdevice ; Solid phase DNA extraction ; Polymerase chain reaction ; Capillary electrophoresis ; Mini Y STR typing
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：78
- 期：Complete
- 页码：489-496
- 全文大小：2173 K
文摘
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We developed an integrated slidable SPE-μPCR-μCE microchip for forensic genotyping.
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The slidable chip enables to get rid of microvalves and tubing for fluidic control.
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Mini Y STR profiles could be identified from 1 μL human whole blood within 60 min.
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Mini Y STR typing could be executed with the female and male blood mixture sample.
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