TIMP2 deficient mice develop accelerated osteoarthritis via promotion of angiogenesis upon destabilization of the medial meniscus

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• 作者：Meng Mi ; Shanshan Shi ; Tianfang Li ; Jonathan Holz ; Yi-Jang Lee ; Tzong-jen Sheu ; Qi ; e Liao ; Tao Xiao
• 关键词：TIMPs ; tissue inhibitor of metalloproteinases ; OA ; osteoarthritis ; DMM ; destabilization of the medial meniscus ; MMPs ; matrix metalloproteinases ; VEGFs ; vascular endothelial growth factors ; HIF ; hypoxia-inducible factors
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 出版时间：29 June, 2012
• 年：2012
• 卷：423
• 期：2
• 页码：366-372
• 全文大小：1521 K

文摘

Vascular invasion into the normally avascular articular surface is a hallmark of advanced osteoarthritis (OA). In this study, we demonstrated that the expression of tissue inhibitor of metalloproteinases-2 (TIMP2), an anti-angiogenic factor, was present at high levels in normal articular chondrocytes, and was drastically decreased shortly after destabilization of the medial meniscus (DMM). We also investigated the anti-angiogenic properties of TIMP2 via knockout. We hypothesized that the loss of TIMP2 could accelerate osteoarthritis development via promotion of angiogenesis. Loss of TIMP2 led to increased periarticular vascular formation 1 month post DMM, compared to wild-type mice, and did so without altering the expression pattern of matrix metalloproteinases and vascular endothelial growth factors. The increased vascularization eventually resulted in a severe degeneration of the articular surface by 4 months post DMM. Our findings suggest that reduction of TIMP2 levels and increased angiogenesis are possible primary events in OA progression. Inhibiting or delaying angiogenesis by TIMP2 expression or other anti-angiogenic therapies could improve OA prevention and treatment.