Isolation of an acyl-glucose-dependent anthocyanin 7-O-glucosyltransferase from the monocot Agapanthus africanus

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• 作者：Taira Miyahara ; Mariko Takahashi ; Yoshihiro Ozeki ; Nobuhiro Sasaki
• 关键词：AAGT ; acyl-glucose-dependent anthocyanin glucosyltransferase ; ANS ; anthocyanidin synthase ; CHS ; chalcone synthase ; GH1 ; glycoside hydrolase family 1 ; Cy3G ; cyanidin 3-glucoside ; Cy3 ; 7dG ; cyanidin 3 ; 7-diglucoside ; F3H ; flavanone 3-hydroxylase ; FG ; feruloyl
• 刊名：Journal of Plant Physiology
• 出版年：2012
• 出版时间：1 September, 2012
• 年：2012
• 卷：169
• 期：13
• 页码：1321-1326
• 全文大小：679 K

文摘

A cDNA encoding an acyl-glucose-dependent anthocyanin 7-O-glucosyltransferase (AaAA7GT) was isolated from Agapanthus africanus petals; this is the first AAGT identified in a monocot. Peak expression of AaAA7GT in developing A. africanus petals occurred before the flowering stage, and was later than found previously for other anthocyanin biosynthetic genes. Analysis of recombinant proteins showed AaAA7GT had strict substrate preference for anthocyanidin 3-O-glycosides. The AaAA7GT amino acid had high sequence similarity to glycoside hydrolase family 1 (GH1) proteins, which typically act as ¦Â-glycosidases. A phylogenetic analysis of amino acid sequences suggested that AAGTs were derived from glycosidase early in the angiosperm lineage.