Dietary administration of a Gracilaria tenuistipitata extract produces protective immunity of white shrimp Litopenaeus vannamei in response to ammonia stress

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• 作者：Yu-Yuan Chen ; Su Sing Sim ; Siau Li Chiew ; Su-Tuen Yeh ; Chyng-Hwa Liou ; Jiann-Chu Chen<sup> ; sup><sup>jcchen@mail.ntou.edu.twsup>
• 关键词：Litopenaeus vannamei ; Gracilaria tenuistipitata extract ; Dietary administration ; Ammonia stress ; Immune parameters
• 刊名：Aquaculture
• 出版年：2012
• 出版时间：11 December, 2012
• 年：2012
• 卷：370-371
• 期：Complete
• 页码：26-31
• 全文大小：1193 K

文摘

White shrimp Litopenaeus vannamei fed diets containing the hot-water extract of Gracilaria tenuistipitata at 0 (control), 0.5, 1.0 and 2.0 g kg<sup>? 1sup> for 21 days, were exposed to 5 mg L<sup>? 1sup> ammonia-N (ammonia as nitrogen), and immune parameters, including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), the total hemocyte count (THC), phenoloxidase (PO) activity, respiratory bursts (RBs), superoxide dismutase (SOD) activity, and hemolymph protein level, were examined 6-120 h post-stress. Shrimp fed the control diet with no ammonia stress served as the background control. Results indicated that the immune parameters of shrimp fed the 2.0 g kg<sup>? 1sup> diet had returned to the background values at 12-24 h post-stress, whereas the immune parameters of control shrimp had returned to the background values at 72-¡Ý 120 h post-stress. It was therefore concluded that L. vannamei fed the extract-containing diets at 2.0 g kg<sup>? 1sup> or less exhibited a protective effect against ammonia stress as evidenced by the earlier recovery of immune parameters. Shrimp subjected to ammonia stress showed a greater decrease in PO activity and later recovery to the background value compared to the decrease and recovery of RBs and SOD activity indicating progressive perturbation of the humoral immunity.