Molecular cloning and characterization of LrTLR4, analysis of its inductive expression and associated down-stream signaling molecules following lipopolysaccharide stimulation and Gram-negative bacterial infection

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• 作者：Mrinal Samanta^a ; ¹ ; ^{mrinal.samanta@icar.gov.in} ; Madhubanti Basu^a ; ¹ ; Banikalyan Swain^a ; Mahismita Paichha^a ; Saswati S. Lenka^a ; Surajit Das^b ; Pallipuram Jayasankar^a ; Nikhil Kumar Maiti^a
• 关键词：Labeo rohita ; TLR4 ; Innate immunity ; IL-6 ; IL-8 ; Type I IFN
• 刊名：Fish & Shellfish Immunology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：60
• 期：Complete
• 页码：164-176
• 全文大小：3001 K
• 卷排序：60

文摘

The cDNA of Labeo rohita TLR4 (LrTLR4) consisted of 2729 bp, encoding 823 aa. LrTLR4 was phylogenetically related to common carp TLR4bb and zebrafish TLR4b. LPS-treatment induced LrTLR4, MyD88, TRIF, TRAF6, IL-6, IL-8 and type-I IFN gene expression. In A.hydrophila infection, LrTLR4 gene expression was significantly induced.