QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

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• 作者：Sheng-hui ZHOU^a ; ^{zhoushenghui826@gmail.com} ; Lin FU^a ; Qiu-hong WU^b ; Jiao-jiao CHEN^a ; Yong-xing CHEN^a ; Jing-zhong XIE^a ; Zhen-zhong WANG^c ; Guo-xin WANG^a ; De-yun ZHANG^a ; Yong LIANG^a ; Yan ZHANG^a ; Ming-shan YOU^a ; Rong-qi LIANG^a ; Jun HAN^d ; Zhi-yong LIU^a ; ^b ; ^{zyliu@genetics.ac.cn}
• 关键词：wheat ; pre-harvest sprouting ; quantitative trait loci ; SNP ; TaVp-1A
• 刊名：Journal of Integrative Agriculture
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：16
• 期：2
• 页码：435-444
• 全文大小：480 K
• 卷排序：16

文摘

Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative trait loci (QTL) for PHS resistance were mapped using an available high-density single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) genetic linkage map developed from a 269 recombinant inbred lines (RILs) population of Yanda 1817×Beinong 6. Using phenotypic data on two locations (Beijing and Shijiazhuang, China) in two years (2012 and 2013 harvesting seasons), five QTLs, designated as QPhs.cau-3A.1, QPhs.cau-3A.2, QPhs.cau-5B, QPhs.cau-4A, and QPhs.cau-6A, for PHS (GP) were detected by inclusive composite interval mapping (ICIM) (LOD≥2.5). Two major QTLs, QPhs.cau-3A.2 and QPhs.cau-5B, were mapped on 3AL and 5BS chromosome arms, explaining 6.29–21.65% and 4.36–5.94% of the phenotypic variance, respectively. Precise mapping and comparative genomic analysis revealed that the TaVp-1A flanking region on 3AL is responsible for QPhs.cau-3A.2. SNP markers flanking QPhs.cau-3A.2 genomic region were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection (MAS).