QCM-based aptamer selection and detection of Salmonella typhimurium

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• 作者：Lijun Wang^a ; ^b ; ¹ ; Ronghui Wang^b ; ¹ ; Fang Chen^c ; Tieshan Jiang^d ; Hong Wang^d ; Michael Slavik^d ; Hua Wei^e ; Yanbin Li^b ; ^d ; ^{yanbinli@uark.edu}
• 关键词：Quartz crystal microbalance ; Aptamer ; SELEX ; Detection ; Salmonella typhimurium
• 刊名：Food Chemistry
• 出版年：2017
• 出版时间：15 April 2017
• 年：2017
• 卷：221
• 期：Complete
• 页码：776-782
• 全文大小：1164 K
• 卷排序：221

文摘

A QCM-based SELEX was developed to select ssDNA aptamers against Salmonella typhimurium. The binding behavior was monitored and recorded in real-time by QCM. The selected aptamer was used as a ligand for the development of a QCM-based aptasensor. The aptasensor was able to detect 103 CFU/mL of S. typhimurium within 1 h.