Phosphorylated Codonopsis pilosula polysaccharide could inhibit the virulence of duck hepatitis A virus compared with Codonopsis pilosula polysaccharide

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• 作者：Ke Ming<sup>asup> ; Yun Chen<sup>asup> ; Fangke Yao<sup>asup> ; Jintong Shi<sup>asup> ; Jingjing Yang<sup>asup> ; Hongxu Du<sup>asup> ; Xunyi Wang<sup>bsup> ; Yixuan Wang<sup>asup> ; Jiaguo Liu ; Ph.D<sup>asup><sup> ; sup><sup>liujiaguo@njau.edu.cnsup>
• 关键词：DHAV ; duck hepatitis A virus ; CPPS ; Codonopsis pilosula polysaccharide ; pCPPS ; phosphorylated Codonopsis pilosula polysaccharide ; DEHs ; duck embryonic hepatocytes ; DVH ; duck virus hepatitis ; DMEM ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium ; MM ; maintenance medium ; D-Hank&rsquo ; s ; Dulbecco&rsquo ; s Hanks balanced salt solution ; MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5- diphenyltetrazolium bromide ; STPP ; sodium tripolyphosphate ; STMP ; sodium trimetaphosphate ; PBS ; phosphate-buffered saline ; CC ; ce
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：94
• 期：part_PA
• 页码：28-35
• 全文大小：1096 K
• 卷排序：94

文摘

To screen effective anti-duck hepatitis A virus (DHAV) drugs, we applied STMP-STPP method to prepare phosphorylated Codonopsis pilosula polysaccharide (pCPPS), the phosphorylation-modified product of Codonopsis pilosula polysaccharide (CPPS). The IR spectrum and field emission scanning electron microscope (FE-SEM) were subsequently used to analyze the structure of pCPPS. Several tests were conducted to compare the anti-DHAV activities of CPPS and pCPPS. The MTT method was used to compare the effect of the drugs on DHAV-infected duck embryonic hepatocytes (DEHs), and the Reed-Muench assay was employed to observe changes in the virulence of DHAV. We also applied real-time PCR to examine the relationship between virus replication and the expression of IFN-β. The results indicated that CPPS could not inhibit the replication of DHAV. In contrast, pCPPS increased the virus TCID50, inhibited viral replication and, accordingly, increased the survival rate of DEHs infected with DHAV. Because DHAV induced the expression of IFN-β, and the IFN-β expression level was positively associated with the number of DHAV, the reduction of IFN-β expression levels after pCPPS treatment demonstrated a decrease in the number of virus particles. These results indicated that pCPPS, which reduces the number of DHAV, was more effective than CPPS in anti-DHAV activity.