Dietary calcium supplementation in adult rats reverts brown adipose tissue dysfunction programmed by postnatal early overfeeding

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• 作者：Ellen Paula Santos Conceiç ; ã ; o^a ; Egberto Gaspar Moura^a ; Elaine Oliveira^a ; Deysla Sabino Guarda^a ; Mariana Sarto Figueiredo^a ; Fernanda Torres Quitete^a ; Camila Calvino^a ; Rosiane Aparecida Miranda^b ; Paulo Cezar Freitas Mathias^b ; Alex Christian Manhã ; es^c ; Patricia Cristina Lisboa^a ; ^{pclisboa@uerj.br}
• 关键词：ADRβ3 ; beta 3 isoform adrenergic receptor ; AgRP ; agouti-related protein ; ANS ; autonomic nervous system ; ARC ; hypothalamic arcuate nucleus ; BAT ; brown adipose tissue ; MCR ; melanocortin receptors ; NL ; normal litter ; POMC ; proopiomelanocortin ; PVN ; hypothalamic paraventricular nucleus ; SL ; small litter ; SL-Ca ; small litter supplemented with calcium carbonate ; TRβ1 ; beta 1 isoform thyroid hormone receptor ; UCP1 ; uncoupling protein 1 ; α-MSH ; alpha-melanocyte-stimulating hormone
• 刊名：The Journal of Nutritional Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：117-125
• 全文大小：1167 K
• 卷排序：39

文摘

Brown adipose tissue (BAT) dysfunction is associated with obesity and its comorbidities, such as hypertension, and the improvement of BAT function seems important for obesity management. Here we investigated the effects of dietary calcium supplementation on BAT autonomic nerve activity, sympathoadrenal function and cardiovascular parameters in adult obese rats that were raised in small litters (SL group). Three days after birth, SL litters were adjusted to three pups to induce early overfeeding. The control group remained with 10 pups/litter until weaning (NL group). At PN120, the SL group was randomly divided into the following: rats fed with standard chow (SL) and rats fed with dietary calcium carbonate supplementation (SL-Ca, 10 g/kg chow). Animals were killed either at PN120 or PN180. At both ages, SL rats had higher BAT autonomic nervous system activity, mass and adipocyte area, as well as increased heart rate and blood pressure (systolic and diastolic); 2 months of calcium supplementation normalized these parameters. At PN180 only, UCP1 and TRβ1 in BAT were decreased in SL rats. These changes were also prevented by calcium treatment. Also at PN180, the SL group presented higher tyrosine hydroxylase and adrenal catecholamine contents, as well as lower hypothalamic POMC and MC4R contents. Calcium supplementation did not revert these alterations. Thus, we demonstrated that dietary calcium supplementation was able to improve cardiovascular parameters and BAT thermogenesis capacity in adult animals that were early overfed during lactation.