Exogenous fatty acids and niacin on acute prostaglandin D₂ production in human myeloid cells

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• 作者：Sergio Montserrat-de la Paz^a ; ^{delapaz@us.es} ; Beatriz Bermudez^b ; Sergio Lopez^a ; Maria C. Naranjo^a ; Yolanda Romero^a ; Maria J. Bando-Hidalgo^a ; Rocio Abia^a ; Francisco J.G. Muriana^a ; ^{muriana@ig.csic.es}
• 关键词：Niacin ; Exogenous fatty acids ; Postprandial state ; Prostaglandin D2 ; Myeloid cells
• 刊名：The Journal of Nutritional Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：22-31
• 全文大小：1366 K
• 卷排序：39

文摘

Niacin activates HCA2 receptor that results in the release of PGD2. However, little is known on PGD2-producing cells and the role of fatty acids. Notably M-CSF macrophages exhibited a timely dependent PGD2 production upon niacin challenge. Short pretreatment of M-CSF macrophages with autologous postprandial TRLs induced the down-regulation of HCA2 gene and up-regulation of genes encoding COX1 and COX2 enzymes in a fatty acid-dependent manner. These effects were paralleled by a higher PGD2 production with postprandial TRL-SFAs. The niacin-mediated transcriptional activity of all genes involved in PGD2 biosynthesis was desensitized in a time-dependent manner by postprandial TRLs, leading to a decreased PGD2 release. In vivo, the net excursions of PGD2 in plasma followed similar fatty acid-dependent patterns as those found for PGD2 release in vitro. The predominant fatty acid class in the diet acutely modulates PGD2 biosynthetic pathway both in vitro and in vivo.