Silencing of BnTT1 family genes affects seed flavonoid biosynthesis and alters seed fatty acid composition in Brassica napus

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• 作者：Jianping Lian^a ; ^b ; ^c ; Xiaochun Lu^a ; ^b ; ^c ; Nengwen Yin^a ; ^b ; ^c ; Lijuan Ma^a ; ^b ; ^c ; Jing Lu^a ; ^b ; ^c ; Xue Liu^a ; ^b ; ^c ; Jiana Li^a ; ^b ; ^c ; Jun Lu^a ; ^b ; ^c ; Bo Lei^a ; ^b ; ^c ; Rui Wang^a ; ^b ; ^c ; Yourong Chai^a ; ^b ; ^c ; ^{chaiyourong2@163.com}
• 关键词：TT1 ; TRANSPARENT TESTA1 ; RNAi ; RNA interference ; PA ; proanthocyanidin ; PC ; procyanidin oligomer ; DP ; degree of polymerization of the epicatechin unit ; tt ; transparent testa ; WT ; wild type ; TT1-RNAi & R ; RNAi transgenic plants harboring pFGC5941M-BTT1I ; DAP ; days after pollination ; qRT-PCR ; quantitative real-time PCR ; TBO ; toluidine blue O ; DEG ; differentially expressed gene ; GO ; Gene Ontology ; KEGG ; Kyoto Encyclopedia of Genes and Genomes ; GC ; gas chromatography ; NIRS ; near-infrared reflectance
• 刊名：Plant Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：254
• 期：Complete
• 页码：32-47
• 全文大小：4524 K
• 卷排序：254

文摘

Silencing of BnTT1 genes altered the seed color, seed size and seed weight in B. napus. Transgenic seeds showed down-regulated expression of genes involved in flavonoid biosynthesis. Silencing of BnTT1 genes decreased flavonols and proanthocyanidins in B. napus. Repression of BnTT1 genes altered the expression of genes involved in fatty acid biosynthesis. Repression of BnTT1 genes altered the seed fatty acid composition.