Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response

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• 作者：Robert Heler¹ ; Addison V. Wright² ; Marija Vucelja³ ; David Bikard¹ ; ⁹ ; Jennifer A. Doudna² ; ⁴ ; ⁵ ; ⁶ ; ⁷ ; ⁸ ; Luciano A. Marraffini¹ ; ¹⁰ ; ^{marraffini@rockefeller.edu}
• 关键词：CRISPR-Cas ; Cas9 ; spacer acquisition ; adaptation ; bacteriophage ; adaptive immunity ; horizontal gene transfer
• 刊名：Molecular Cell
• 出版年：2017
• 出版时间：5 January 2017
• 年：2017
• 卷：65
• 期：1
• 页码：168-175
• 全文大小：2070 K
• 卷排序：65

文摘

A library of cas9 alleles was screened for clones with enhanced CRISPR-Cas immunity The I473F substitution in Cas9 enhances the CRISPR immune response by 100-fold This phenotype is due to an increase of the rate of spacer acquisition Cas9 is involved in spacer acquisition, and the I473F mutation is a tool to study it