Conformational Freedom of the LRP6 Ectodomain Is Regulated by N-glycosylation and the Binding of the Wnt Antagonist Dkk1

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• 作者：Kyoko Matoba¹ ; Emiko Mihara¹ ; Keiko Tamura-Kawakami¹ ; Naoyuki Miyazaki¹ ; Shintaro Maeda¹ ; Hidenori Hirai¹ ; Samuel Thompson¹ ; ² ; Kenji Iwasaki¹ ; Junichi Takagi¹ ; ³ ; ^{takagi@protein.osaka-u.ac.jp}
• 关键词：LRP6 ; Wnt signal ; negative-stain electron microscopy ; Dkk1 ; single particle analysis ; N-glycosylation ; conformational flexibility ; receptor ectodomain
• 刊名：Cell Reports
• 出版年：2017
• 出版时间：3 January 2017
• 年：2017
• 卷：18
• 期：1
• 页码：32-40
• 全文大小：2591 K
• 卷排序：18

文摘

Negative-stain EM reveals that the entire LRP6 ectodomain is flexible Bending angle of the LRP6 ectodomain is regulated by N-glycans located at the hinge 3D structure of LRP6ec/Dkk1 complex is determined by the random conical tilt method Dkk1 binding induces conformational changes in the LRP6 ectodomain