New wrinkling substrate assay reveals traction force fields of leader and follower cells undergoing collective migration

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• 作者：Sho Yokoyama^a ; ¹ ; Tsubasa S. Matsui^a ; ^b ; Shinji Deguchi^a ; ^b ; ^{deguchi@me.es.osaka-u.ac.jp}
• 关键词：Collective cell migration ; Epithelial cells ; MDCK-II cells ; Traction force microscopy ; Wrinkling substrate assay
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：975-979
• 全文大小：1614 K
• 卷排序：482

文摘

Substrates highly sensitive to detect cellular contractile forces are developed. Leader cells do not affect the speed of collective migration of MDCK-II cells. Leader cells exhibit significant traction forces and tend to undergo apoptosis. Traction force fields in cell colonies are determined by local individual cells. Marginal cells in colonies have spread morphology to exhibit high traction forces.