Cyclosporine A inhibits TGF-β2-induced myofibroblasts of primary cultured human pterygium fibroblasts

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• 作者：Sang Il Gum^a ; ¹ ; Yeoun-Hee Kim^a ; ^b ; ¹ ; Jae-Chang Jung^c ; Im Gyu Kim^a ; Jun Seok Lee^a ; Kyoo Won Lee^a ; Young Jeung Park^a ; ^{eyepark9@naver.com}
• 关键词：Cyclosporine A ; TGF-β2 ; Myofibroblasts ; Pterygium fibroblasts ; α-SMA ; Laminin ; Fibronectin
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：1148-1153
• 全文大小：1249 K
• 卷排序：482

文摘

Cyclosporine A (CsA), an immunomodulatory drug, and is increasingly used to treat moderate dry eye syndrome and ocular surface inflammation. However, any inhibitory effect on differentiation of fibroblasts to myofibroblasts remains unclear. Here, we show that the inhibitory effect of CsA on transforming growth factor-beta2 (TGF-β2)-induced myofibroblasts in primary cultured human pterygium fibroblasts. CsA significantly decreased mRNA and protein expression of myofibroblast-related markers including α-SMA, laminin, and fibronectin. These findings were supported by the results from immunofluorescence staining. Taken together, these results indicate the therapeutic potential of CsA against pterygium progression. Further studies are necessary to elucidate the precise intracellular signal mechanism responsible for CsA-induced downregulation of myofibroblast markers in pterygium fibroblasts.