Functional characterization of PhoPR two component system and its implication in regulating phosphate homeostasis in Bacillus anthracis

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• 作者：Somya Aggarwal^a ; ¹ ; Vikas Kumar Somani^a ; ¹ ; Vatika Gupta^a ; Jaspreet Kaur^b ; ² ; Damini Singh^a ; Abhinav Grover^b ; Rakesh Bhatnagar^a ; ^{rakeshbhatnagar@jnu.ac.in}
• 关键词：TCS ; two component system ; HK ; histidine kinase ; RR ; response regulator ; PS ; phosphate starvation ; AcP ; acetyl phosphate ; MBP ; maltose binding protein ; ELISA ; enzyme linked immunosorbent assay ; AP ; alkaline phosphatase ; HRP ; horse radish peroxidase ; EMSA ; electrophoretic mobility shift assay ; MSA ; multiple sequence alignment
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1861
• 期：1
• 页码：2956-2970
• 全文大小：3133 K
• 卷排序：1861

文摘

Phosphate starvation (PS) promotes pathogenesis of B. anthracis (BA) within host. PhoP and PhoR of BA form a functional two component system, regulating PS response. PhoP is implicated in regulation of PS marker genes. H370 of PhoR and D10, D53 and M55 of PhoP are involved in PhoPR interaction. Exclusive in microbes, homology in pathogens, role in virulence & PS make PhoP as a good drug target.