High temperature and UV-C treatments affect stilbenoid accumulation and related gene expression levels in Gnetum parvifolium

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• 作者：Nan Deng^a ; ¹ ; Caixia Liu^a ; ¹ ; Ermei Chang^a ; Jing Ji^a ; Xiamei Yao^a ; Jianyun Yue^a ; Igor V. Bartish^b ; Lanzhen Chen^c ; ^d ; Zeping Jiang^a ; ^{jiangzp@caf.ac.cn} ; Shengqing Shi^a ; ^{shi.shengqing@caf.ac.cn}
• 关键词：Antibacterial ; Anticancer ; Antioxidative ; Chinese medicine ; Gnetophyta ; Natural bioactive compounds ; Polyphenols ; Real-time PCR ; Seedlings ; Stilbenes ; Stress-treated roots
• 刊名：Electronic Journal of Biotechnology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：25
• 期：Complete
• 页码：43-49
• 全文大小：631 K
• 卷排序：25

文摘

Gnetum parvifolium stems and roots have been used for a long time in traditional Chinese medicines. Stilbenes are bioactive compounds present in G. parvifolium plants, and they possess antioxidative and anticancer properties. However, little is known about the responses of G. parvifolium stilbene biosynthetic pathways to stress conditions. Therefore, we investigated stilbene biosynthesis, including the expression of relevant genes, in G. parvifolium exposed to high-temperature and ultraviolet-C treatments.ResultsHigh temperatures did not influence the accumulation of total stilbenes in stems but decreased stilbene concentrations in roots at 3 h, with a subsequent restoration to control levels. In contrast, ultraviolet irradiation induced the accumulation of total stilbenes in stems but not in roots. We also observed that high temperatures inhibited the production of resveratrol and piceatannol in G. parvifolium stems and roots, whereas ultraviolet treatments initially inhibited their accumulation (up to 6 h) but induced their production at later time points. Analyses of specific genes (i.e., PAL, C4H, 4CL, STS, and CYP) revealed that their expression levels generally increased in stress-treated stems and roots, although there was some variability in the expression profiles during treatments.ConclusionsOur results indicated that high temperatures and ultraviolet irradiation differentially affect the biosynthesis of specific stilbenes in G. parvifolium stems and roots. Therefore, cultivating G. parvifolium seedlings under optimal stress conditions may increase the biosynthesis of specific stilbene compounds.