MAPK/ERK activity is required for the successful progression of mitosis in sea urchin embryos

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• 作者：Odile Mulner-Lorillon^a ; ^b ; ^{mulner@sb-roscoff.fr} ; Hé ; loï ; se Chassé ; ^a ; ^b ; Julia Morales^a ; ^b ; Robert Bellé ; ^a ; ^b ; Patrick Cormier^a ; ^b
• 关键词：MAPK/ERK pathway ; Embryonic cell division ; Cell cycle checkpoint
• 刊名：Developmental Biology
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：421
• 期：2
• 页码：194-203
• 全文大小：1360 K
• 卷排序：421

文摘

Using sea urchin embryos, we demonstrate that the MEK/MAPK/ERK cascade is essential for the proper progression of the cell cycle. Activation of a limited fraction of MAPK/ERK is required between S-phase and M-phase. Neither DNA replication nor CDK1 activation are impacted by the inhibition of this small active MAPK/ERK fraction. Nonetheless, the chromatin and spindle organisations are profoundly altered. Early morphological disorders induced by the absence of MAPK/ERK activation are correlated with an important inhibition of global protein synthesis and modification in the cyclin B accumulation profile. After appearance of morphological disorders, there is an increase in the level of the inhibitor of protein synthesis, 4E-BP, and, ultimately, an activation of the spindle checkpoint. Altogether, our results suggest that MAPK/ERK activity is required for the synthesis of (a) protein(s) implicated in an early step of chromatin /microtubule attachment. If this MAPK/ERK-dependent step is not achieved, the cell activates a new checkpoint mechanism, involving the reappearance of 4E-BP that maintains a low level of protein translation, thus saving cellular energy.