Effects of trehalose vitrification and artificial oocyte activation on the development competence of human immature oocytes

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• 作者：Zhiguo Zhang^a ; ^b ; ^c ; ¹ ; Tianjuan Wang^a ; ¹ ; Yan Hao^a ; ¹ ; Fazil Panhwar^d ; ¹ ; Zhongrong Chen^d ; ¹ ; Weiwei Zou^a ; Dongmei Ji^a ; Beili Chen^a ; Ping Zhou^a ; Gang Zhao^d ; ^{ZhaoG@ustc.edu.cn} ; Yunxia Cao^a ; ^{caoyunxia6@126.com}
• 关键词：Trehalose vitrification ; Artificial oocyte activation (AOA) ; Human immature oocytes ; Aneuploidy ; Fluorescence in situ hybridization (FISH)
• 刊名：Cryobiology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：74
• 期：Complete
• 页码：43-49
• 全文大小：628 K
• 卷排序：74

文摘

Sucrose and trehalose are conventional cryoprotectant additives for oocytes and embryos. Ethanol can artificially enhance activation of inseminated mature oocytes. This study aims to investigate whether artificial oocyte activation (AOA) with ethanol can promote the development competence of in vitro matured oocytes. A total of 810 human immature oocytes, obtained from 325 patients undergoing normal stimulated oocyte retrieval cycles, were in vitro maturated (IVM) either immediately after collection (Fresh group n = 291)) or after being vitrified as immature oocytes (Vitrified group n = 519). These groups were arbitrarily assigned. All fresh and vitrified oocytes which matured after a period of IVM then underwent intra-cytoplasmic sperm injection (ICSI). Half an hour following ICSI, they were either activated by 7% ethanol (AOA group) or left untreated (Non-AOA group). Fertilization, cleavage rate, blastocyst quality and aneuploidy rate were then evaluated. High-quality blastocysts were only obtained in both the fresh and vitrified groups which had undergone AOA after ICSI. Trehalose vitrification slightly, but not significantly, increased the formation rates of high-quality embryos (21.7% VS 15.4%, P > 0.05) and blastocysts (15.7% VS 7.69%, P > 0.05)) when compared with sucrose vitrification. Aneuploidy was observed in 12 of 24 (50%) of the AOA derived high quality blastocysts. High-quality blastocysts only developed from fresh or vitrified immature oocytes if the ICSI was followed by AOA. This information may be important for human immature oocytes commonly retrieved in normal stimulation cycles and may be particularly important for certain patient groups, such as cancer patients. AOA with an appropriate concentration of ethanol can enhance the developmental competence of embryos.