Resolving interspecific relationships within evolutionarily young lineages using RNA-seq data: An example from Pedicularis section Cyathophora (Orobanchaceae)
Flower transcriptome was sequenced for all Pedicularis sect. Cyathophora species. Interspecific relationships of sect. Cyathophora are robustly reconstructed. All genes with different Ka/Ks values can be used for phylogenomic reconstruction. Species phylogeny of sect. Cyathophora can be resolved by ⩾20–25 single-/low-copy genes.