TTHA0596 is a metal binding protein belonging to cluster A-I family of SBPs. Evolutionarily, TTHA0596 is closer to Zn2 + and Mn2 + binding SBPs rather than Fe2 +/3 + binding SBPs. TTHA0596 belongs to group I Zn2 + binding SBPs which exhibit additional affinity for Mn2 + ions. TTHA0596 protein may bind both Zn2 + and Mn2 + ions.