Application of modified yeast surface display technologies for non-Antibody protein engineering

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• 作者：Meng Mei ; Yu Zhou ; Wenfang Peng ; Chan Yu ; Lixin Ma ; Guimin Zhang ; ^{zhangguimin6@hotmail.com} ; Li Yi ; ^{liyi@hubu.edu.cn}
• 关键词：Yeast surface display ; FACS ; Directed evolution ; Non-antibody protein engineering
• 刊名：Microbiological Research
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：196
• 期：Complete
• 页码：118-128
• 全文大小：1599 K
• 卷排序：196

文摘

Yeast surface display (YSD) system has been widely used in protein engineering since it was established 20 years ago. Combined with fluorescence-activated cell sorting (FACS) technology and directed evolution, YSD has been proven of its extraordinary effectiveness for molecular engineering of various target proteins, especially for antibodies. Recently, a few remarkable efforts were exploited to modify the original Aga1-Aga2 YSD for the non-antibody protein engineering with successful outcomes, expanding its application on oxidase, Class II major histocompatibility complex (MHC-II), protease, sortase, lipoic acid ligase etc. Here, the methodologies of these optimized Aga1-Aga2 YSD technologies were introduced, and the recent progress of non-antibody protein engineering using these methods was summarized.