Separation of human Fab fragments by negative chromatography on ω-aminohexyl- and poly(ethyleneimine)-agarose

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• 作者：Gabriela Pannunzio Carmignotto ; Cec&iacute ; lia Alves Mourã ; o ; Sonia Maria Alves Bueno ; ^{sonia@feq.unicamp.br}
• 关键词：Human fab fragments ; Aminohexyl ; Poly(ethyleneimine) ; Purification ; Adsorption ; Negative chromatography
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：52
• 期：Complete
• 页码：267-275
• 全文大小：1502 K
• 卷排序：52

文摘

Evaluate polyamines as adsorbents for human Fab fragment purification. Fab fragments were obtained by negative chromatography on polyamine-agarose. Fc fragment adsorption occurred by electrostatic and hydrophobic interactions. Fab and Fc fragments were separated on polyamine-agarose due to their different pI.