Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of recombinant thermostable alkylsulfatase (SdsAP) from Pseudomonas sp. S9

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• 作者：Shengping Li^a ; Yintao Su^a ; Yadan Liu^a ; Lifang Sun^a ; Minxiang Yu^b ; Yunkun Wu^a ; ^{wuyk@fjirsm.ac.cn}
• 关键词：Isopropanol (PubChem CID ; 3776) ; Tert-butanol (PubChem CID ; 6386) ; Acetonitrile (PubChem CID ; 6342) ; Ammonium sulfate (PubChem CID ; 6097028) ; PEG4000 (PubChem CID ; 448032) ; Glutaraldehyde (PubChem CID ; 3485) ; Dichloromethane (PubChem CID ; 6344) ; DMF (PubChem CID ; 6228) ; Chloroform (PubChem CID ; 6212) ; DMSO (PubChem CID ; 679)
• 刊名：Process Biochemistry
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：51
• 期：12
• 页码：2084-2089
• 全文大小：1276 K
• 卷排序：51

文摘

SdsAP, an alkylsulfatase for SDS degradation, was immobilized as CLEAs with high activity. SdsAP-CLEAs showed higher pH and temperature stability, and better tolerance to a certain organic solvents. SdsAP-CLEAs had higher affinity and catalytic efficiency than its soluble counterpart. SdsAP-CLEAs also showed good reusability and storage resistance. The stability and operability of SdsAP-CLEAs exhibiting a great potential application of on SDS degradation in industry wastewater treatment.