Osteogenic differentiation capacity of human mesenchymal stromal cells in response to extracellular calcium with special regard to connexin 43

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• 作者：Alena-Svenja Wagner^a ; ^{Alena-Svenja.Wagner@vetmed.uni-giessen.de} ; Kristina Glenske^a ; Verena Wolf^a ; Daniela Fietz^b ; Sybille Mazurek^c ; Thomas Hanke^d ; Andreas Moritz^e ; Stefan Arnhold^b ; Sabine Wenisch^a
• 关键词：BM ; basal medium [α-MEM (Invitrogen-Life Technologies ; Darmstadt) ; 10% FBS (Biochrome ; Berlin ; Germany) and 100&#xA0 ; U/ml penicillin/streptomycin (PAA Laboratories ; Pasching ; Austria)] ; OM ; osteogenic medium [α-MEM (Invitrogen-Life Technologies ; Darmstadt) ; 10% FBS (Biochrome ; Berlin ; Germany) and 100&#xA0 ; U/ml penicillin/streptomycin (PAA Laboratories ; Pasching ; Austria)&#xA0 ; +&#xA0 ; 10&#xA0 ; mmol&#xA0 ; l&minus ; 1 β-glycerophosphate ; 0.06&#xA0 ; mmol&#xA0 ; l&minus ; 1 ascorbic acid ; and 0.1&#xA0 ; μmol
• 刊名：Annals of Anatomy - Anatomischer Anzeiger
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：209
• 期：Complete
• 页码：18-24
• 全文大小：1917 K
• 卷排序：209

文摘

The effects of extracellular calcium on osteogenic differentiation capacity of human bone-derived mesenchymal stromal cells with special regard to connexin 43 (cx43) have been investigated by means of cell culture experiments. Mesenchymal stromal cells isolated from human cancellous bone were cultured on tissue culture plates at different calcium ion (Ca2+) concentrations (1.8 mmol l−1, 10 mmol l−1, 20 mmol l−1). Cell responses were evaluated by quantitative RT-PCR, immunofluorescence staining, and Lucifer Yellow fluorescence uptake experiments. It could be shown that increasing Ca2+ concentrations correlate with increasing cx43 and bone sialoprotein mRNA levels as well as with enhanced cx43 fluorescence signaling and matrix mineralization of the cultures as shown by von Kossa staining. Hemichannel gating – assessed by Lucifer Yellow uptake – increases with increasing extracellular Ca2+ concentrations suggesting that regulatory effects at the hemichannel level are calcium-dependent.