JAK family members: Molecular cloning, expression profiles and their roles in leptin influencing lipid metabolism in Synechogobius hasta

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• 作者：Kun Wu^a ; Xiao-Ying Tan^a ; ^b ; ^{txy7933@163.com} ; ^{txy7933@mail.hzau.edu.cn} ; Yi-Huan Xu^a ; Xi Shi^a ; Yao-Fang Fan^a ; Dan-Dan Li^a ; Xu Liu^c
• 关键词：ACC ; acetyl-CoA carboxylase ; ATGL ; adipose triglyceride lipase ; FAS ; fatty acid synthase ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; HPRT ; hypoxanthine-guanine phosphoribosyl transferase ; HSL ; hormone-sensitive lipase ; JAK ; janus kinase ; LepR ; leptin receptor ; ME ; malic enzyme ; MS-222 ; tricaine methanesulfonate ; RPL7 ; ribosomal protein L7 ; STAT ; signal transducer and activator of transcription ; TBP ; TATA-box-binding protein ; TG ; triglyceride ; TUBA ; tubulin alpha chain ; UBCE ; ubiquitin-conj
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：203
• 期：Complete
• 页码：122-131
• 全文大小：4900 K
• 卷排序：203

文摘

Janus kinase (JAK) is a family of non-receptor tyrosine kinases that participate in transducing cytokine signals from the external environment to the nucleus in various biological processes. Currently, information about their genes structure and evolutionary history has been extensively studied in mammals as well as in several fish species. By contrast, limited reports have addressed potential role of diverse JAK in signaling responses to leptin in fish. In this study, we identified and characterized five JAK members of Synechogobius hasta. Compared to mammals, more members of the JAK family were found in S. hasta, which provided evidence that the JAK family members had arisen by the whole genome duplications during vertebrate evolution. For protein structure, all of these members possessed similar domains compared with those of mammals. Their mRNAs were expressed in a wide range of tissues, but at the different levels. Incubation in vitro of freshly isolated hepatocytes of S. hasta with different concentrations of recombinant human leptin decreased the intracellular triglyceride content and lipogenic genes expression, and increased mRNA expression of several JAK and lipolytic genes. AG490, a specific inhibitor of JAK, reversed leptin-induced effects on TG content and JAK2a, JAK2b, hormone-sensitive lipase (HSL2) and acetyl-CoA carboxylase (ACCa), indicating that the JAK2a/b may have mediated the actions of leptin on lipid metabolism at transcriptional level.