Deletion of the vacJ gene affects the biology and virulence in Haemophilus parasuis serovar 5

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• 作者：Liangyou Zhao^a ; ^b ; Xueli Gao^a ; Chaonan Liu^a ; Xiaoping Lv^a ; Nan Jiang^c ; Shimin Zheng^a ; ^{zhengshiminbl@sohu.com}
• 关键词：NAD ; nicotinamide-adenine dinucleotide ; LB ; Luria-Bertani ; TSA ; Tryptic Soy Agar ; TSB ; Tryptic Soy Broth ; OD600 ; optical density at 600  ; nm ; Kan ; kanamycin ; MIC ; minimal inhibitory concentration ; LD50 ; 50% lethal dose ; i.p. ; intraperitoneally ; ORF ; open reading frame
• 刊名：Gene
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：603
• 期：Complete
• 页码：42-53
• 全文大小：1360 K
• 卷排序：603

文摘

Haemophilus parasuis VacJ lipoprotein was implicated in maintaining the outer membrane integrity. Deleting the vacJ gene increased susceptibility to stress conditions, several antibiotics and complement-dependent killing. Deleting the vacJ gene decreased ability to biofilm formation, adherence to and invasion of PK-15 cells. Deleting the vacJ gene attenuated the pathogenicity of H. parasuis in mice.