Identification and evaluation of reference genes for expression studies by RT-qPCR during embryonic development of the emerging model organism, Macrobrachium olfersii

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• 作者：Michael L. Jaramillo^a ; ¹ ; Dib Ammar^a ; ^b ; ¹ ; Ruth L. Quispe^c ; Frank Guzman^d ; Rogerio Margis^e ; Evelise M. Nazari^a ; ² ; ^{evelise.nazari@ufsc.br} ; Yara M.R. M&uuml ; ller^a ; ²
• 关键词：RT-qPCR ; real-time reverse transcription quantitative polymerase chain reaction ; cDNA ; complementary DNA ; MIQE ; Minimum Information for Publication of Quantitative Real-Time PCR Experiments ; β-act ; β-actin ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; RpL8 ; ribosomal protein L8 ; RpS6 ; ribosomal protein S6 ; EF-1α ; elongation factor-1α ; AK ; arginine kinase ; M ; muscle ; HPT ; hepatopancreas ; CG ; cerebral ganglia ; Cq ; quantification cycle ; Ct ; threshold cycle ; E ; efficiency ; NFs ; normalization fac
• 刊名：Gene
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：598
• 期：Complete
• 页码：97-106
• 全文大小：1276 K
• 卷排序：598

文摘

Six reference genes of M. olfersii were identified and evaluated. RpL8 and RpS6 were the most stable genes in embryonic developmental and adult tissues analyzed. The EF-1α and GAPDH genes were unsuitable for RT-qPCR normalization of M. olfersii developmental stages due to its expression variability. Impact of normalization with various reference genes was demonstrated during embryonic stages.