Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

详细信息    查看全文

• 作者：Nadine Alvarez¹ ; Daymí ; Serpa¹ ; Ramlah Kadir² ; Yanely Tirado¹ ; Reinier Borrero¹ ; Sonsire Ferná ; ndez¹ ; Rubé ; n Cabrera¹ ; Yolanda Valdes¹ ; Caridad Zayas¹ ; Reinaldo Acevedo¹ ; Luis Izquierdo¹ ; Marí ; a Elena Sarmiento² ; Mohd-Nor Norazmi² ; ³ ; ^{norazmimn@usm.my} ; José ; Luis Pé ; rez¹ ; Armando Acosta³ ; ^{ducmar13@gmail.com}
• 关键词：BCG ; Immunogenicity ; Mycobacterium tuberculosis ; Proteoliposomes
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：7
• 期：3
• 页码：188-192
• 全文大小：604 K
• 卷排序：7

文摘

To characterize the immunogenicity and the induction of cross-reactive responses against Mycobacterium tuberculosis (M. tuberculosis) of a proteoliposome (PL) from Mycobacterium bovis Bacillus Calmette–Guérin (BCG) with and without alum hydroxide (AL) as adjuvant (PLBCG-AL and PLBCG, respectively) in BALB/c mice.MethodsBALB/c mice were inoculated with phosphate buffer solution, BCG, PLBCG and PLBCG-AL. The humoral immunogenicity was determined by ELISA [immunoglobulin G (IgG), IgG1 and IgG2a] and the cellular immunogenicity was evaluated in vivo by delayed type hypersensitivity. The humoral cross-reactive response against M. tuberculosis was determined by Western blot.ResultsSera from animals immunized with PLBCG-AL and PLBCG showed significant increase in specific total IgG and IgG1 antibodies and the presence of cross-reactive antibodies against M. tuberculosis antigens, which were more intense with the use of alum as adjuvant. Mice immunized with PLBCG and PLBCG-AL also showed a specific cellular response in vivo.ConclusionsThe cellular and humoral immunogenicity of PLBCG and the capacity to induce cross-reactive responses against M. tuberculosis is in agreement with the protective capacity previously demonstrated by this vaccine candidate and supports the continuation of its evaluation in further stages.