Dual function of Ixr1 in transcriptional regulation and recognition of cisplatin-DNA adducts is caused by differential binding through its two HMG-boxes

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• 作者：A. Vizoso-Vá ; zquez ; M. Lamas-Maceiras ; R. Ferná ; ndez-Leiro¹ ; A. Rico-Dí ; az ; M. Becerra ; M.E. Cerdá ; n ; ^{esper.cerdan@udc.es}
• 关键词：Thermodynamics ; Protein-DNA interactions ; SOX transcriptional factors ; DNA repair ; HMGB proteins ; Saccharomyces cerevisiae
• 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1860
• 期：2
• 页码：256-269
• 全文大小：2006 K
• 卷排序：1860

文摘

HMG-box A and HMG-box B of Ixr1 have specialized functions in the recognition of DNA at regulated promoters HMG-box A binds to regulatory sequences with higher affinity and produces higher DNA bending than HMG-box B In tandem arrangement of HMG-box A and B influences folding, favors DNA binding and is necessary for specific DNA recognition Binding of HMG-box A to specific regulatory sequences of DNA or cisplatin adducts is thermodynamically distinguishable