The shape effect of magnetic mesoporous silica nanoparticles on endocytosis, biocompatibility and biodistribution

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• 作者：Dan Shao^a ; ^b ; ^e ; ¹ ; Meng-meng Lu^d ; ^e ; ¹ ; Ya-wei Zhao^a ; Fan Zhang^a ; Yong-fei Tan^a ; Xiao Zheng^a ; Yue Pan^a ; Xuan-ang Xiao^a ; Zheng Wang^a ; ^b ; Wen-fei Dong^b ; ^{wenfeidong@126.com} ; Jing Li^a ; ^{lijing@jlu.edu.cn} ; Li Chen^a ; ^c
• 关键词：Magnetic mesoporous silica nanoparticles ; Shape ; Endocytosis ; Tumor accumulation ; Biodistribution ; Biocompatibility
• 刊名：Acta Biomaterialia
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：49
• 期：Complete
• 页码：531-540
• 全文大小：3070 K
• 卷排序：49

文摘

Although the aspect ratio (AR) play a crucial role in determining biological effects of homogeneous nanomaterials, studies available concerning how the shape contributes to biological effect of heterogeneous nanomaterials is limited. To systematically clarify the shape influence on the endocytosis, biocompatibility and biodistribution of magnetic mesoporous silica nanoparticles (M-MSNPs), three FITC-labeled M-MSNPs with different aspect ratio (AR = 1, 2, and 4) were specifically designed and constructed through altering the ratios of CTAB/TEOS in a modified so-gel method. We have demonstrated that long-rod M-MSNP2 possessed higher intracellular internalization amount than the short-rod M-MSNP1 and the sphere-like M-MSNP0 in both cancer cells and normal cells due to the difference in the endocytosis pathways. However, there are no significant shape effects on biocompatibility including cytotoxicity and hemolytic rate. Moreover, biodistribution in HepG2 tumor-bearing mice showed that M-MSNPs administrated intravenously were mainly presented in reticuloendothelial system (RES) organs including liver, spleen and kidney. In particular, sphere-like M-MSNP0 were easily trapped in the liver, while long-rod M-MSP2 exhibited more retention in the spleen. It is worth noting that rod-like M-MSNPs are preferentially accumulated in tumor sites than sphere-like M-MSNPs, indicating an improved drug delivery efficacy in cancer therapy. Our findings may provide useful data for deeply understanding the interaction between the different shapes and biological behavior of M-MSNPs, which is expected to give rise to a new generation of heterogeneous M-MSNPs with significantly enhanced efficacy and safety for the cancer theranostics.Statement of SignificanceIn this work, we systematically clarified the shape influence on the endocytosis, biocompatibility and biodistribution of homogeneous nanomaterials. We have demonstrated that rod-like magnetic mesoporous silica nanoparticles (M-MSNPs) were capable of higher intracellular internalization and tumor accumulation than sphere-like M-MSNPs, which was expected to give rise to a new generation of heterogeneous M-MSNPs with significantly enhanced efficacy and safety for the cancer theranostics.