Chronic exposure of zinc oxide nanoparticles causes deviant phenotype in Drosophila melanogaster

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• 作者：Avnika Singh Anand ; Dipti N. Prasad ; Shashi Bala Singh ; Ekta Kohli ; ^{ektakohli@hotmail.com} ; ^{ektakohli@dipas.drdo.in}
• 关键词：ROS ; reactive oxidative species ; ZnO ; zinc oxide ; AgNPs ; silver nanoparticles ; AuNPs ; gold nanoparticles ; NPs ; nanoparticles ; NMs ; nanomaterials ; TEM ; transmission electron microscopy ; DCF-DA ; 2&rsquo ; 7&rsquo ; &ndash ; dichlorofluorescin diacetate ; DAPI ; 4' ; 6-diamidino-2-phenylindole ; TUNEL ; terminal deoxynucleotidyl transferase dUTP nick end labelling ; ANOVA ; analysis of variance
• 刊名：Journal of Hazardous Materials
• 出版年：2017
• 出版时间：5 April 2017
• 年：2017
• 卷：327
• 期：Complete
• 页码：180-186
• 全文大小：1560 K
• 卷排序：327

文摘

Zinc oxide nanoparticles (ZnO NPs) are commonly used nanomaterials (NMs) with versatile applications from high-end technologies to household products. This pervasive utilisation has brought human in the close interface with nanoparticles (NPs), hence questioning their safety prior to usage is a must. In this study, we have assessed the effects of chronic exposure to ZnO NPs (<50 nm) on the model organism Drosophila melanogaster. Potential toxic effects were studied by evaluating longevity, climbing ability, oxidative stress and DNA fragmentation. Ensuing exposure, the F0 (parent), F1, F2, F3 and F4 generation flies were screened for the aberrant phenotype. Flies exposed to ZnO NPs showed distinctive phenotypic changes, like deformed segmented thorax and single or deformed wing, which were transmitted to the offspring’s in subsequent generations. The unique abnormal phenotype is evident of chronic toxicity induced by ZnO NPs, although appalling, it strongly emphasize the importance to understand NPs toxicity for safer use.