Efficient production of ethyl (R)-4-chloro-3-hydroxybutanoate by a novel alcohol dehydrogenase from Lactobacillus curieae S1L19

详细信息    查看全文

• 作者：Yiping Zhang ; Hualei Wang ; ^{hlwang@ecust.edu.cn} ; Lifeng Chen ; Kai Wu ; Jingli Xie ; Dongzhi Wei
• 关键词：Cofactor ; Co-expression ; Asymmetric reduction ; Ethyl-(R)-4-chloro-3-hydroxybutanoate
• 刊名：Journal of Molecular Catalysis B: Enzymatic
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：134
• 期：part_PA
• 页码：51-60
• 全文大小：1495 K
• 卷排序：134

文摘

A novel dehydrogenase (LCRIII) was heterologously overexpressed in Escherichia coli. An efficient cofactor regeneration system was built by coexpressing two genes in E. coli cells. The asymmetric reduction reaction was conducted at high substrate loading (246.8 g/L). The process could be performed even without external addition of cofactors.