Metabolic engineering of Corynebacterium glutamicum for the production of 3-hydroxypropionic acid from glucose and xylose

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• 作者：Zhen Chen^a ; ^b ; ^{zhenchen2013@mail.tsinghua.edu.cn} ; Jinhai Huang^a ; Yao Wu^a ; Wenjun Wu^a ; Ye Zhang^a ; Dehua Liu^a ; ^b
• 关键词：3-Hydroxypropionic acid ; Corynebacterium glutamicum ; Metabolic engineering ; Glycerol pathway ; Lignocellulose
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：151-158
• 全文大小：811 K
• 卷排序：39

文摘

Corynebacterium glutamicum was engineered for the first time to produce 3-hydroxypropionic acid (3-HP) from glucose via glycerol pathway. 62.6 g/L of 3-HP was produced from glucose at a yield of 0.51 g/g, the highest titer and yield of 3-HP from sugar reported to date. Downregulation of glyceraldehyde 3-phosphate dehydrogenase and employment of the non-PTS glucose-uptake system are important for increasing the yield of 3-HP. 3-HP overproduction by simultaneous utilization of glucose and xylose was achieved.