Myricitrin protects against peroxynitrite-mediated DNA damage and cytotoxicity in astrocytes

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• 作者：Wei Chen^a ; ^b ; ^{zjuchenwei@zju.edu.cn} ; Jingjing Zhuang^b ; Ya Li^b ; Yang Shen^b ; Xiaodong Zheng^a ; ^{xdzheng@zju.edu.cn}
• 关键词：MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; ROS ; reactive oxygen species ; DMPO ; 5 ; 5-dimethylpyrroline-N-oxide ; DMPO-OH ; DMPO-hydroxyl adduct ; DMPO-OOH ; DMPO-superoxide spin adduct ; EPR ; electron paramagnetic resonance ; SOD ; superoxi
• 刊名：Food Chemistry
• 出版年：2013
• 出版时间：15 November, 2013
• 年：2013
• 卷：141
• 期：2
• 页码：927-933
• 全文大小：1237 K

文摘

Peroxynitrite, a potent oxidising and nitrating species, has been implicated in the pathogenesis of neurodegenerative diseases. This study was undertaken to investigate the protective effect of myricitrin on peroxynitrite-mediated toxicity and the underlying mechanism. Our results showed that the presence of myricitrin was found to significantly inhibit peroxynitrite-mediated DNA damage. EPR spectroscopy demonstrated that myricitrin potently diminished the DMPO-hydroxyl radical adduct signal from peroxynitrite. Further study showed that glutathione (GSH) depletion caused by peroxynitrite can be effectively prevented by pre-incubation of astrocytes with myricitrin. Moreover, co-incubation of astrocytes with myricitrin and buthionine sulfoximine (BSO) eliminated the myricitrin-induced GSH increase. In contrast, co-incubation of myricitrin with BSO slightly protected astrocytes against cytotoxicity and DNA damage mediated by peroxynitrite. These results revealed that myricitrin can protect against peroxynitrite-induced DNA damage and cytotoxicity, which might have implications for myricitrin-mediated neuroprotection.