Development and evaluation of a removable tissue-engineered muscle with artificial tendons

详细信息    查看全文

• 作者：Tomohiro Nakamura¹ ; ^{tomohiro.nakamura@oit.ac.jp} ; Shunya Takagi² ; ^{d1d13h01@st.oit.ac.jp} ; Takafumi Kamon² ; ^{t-kamon@kawasumi.jp} ; Ken-ichi Yamasaki² ; ^{kenichi.yamasaki@fukuda.co.jp} ; Toshia Fujisato² ; ^{toshiya.fujisato@oit.ac.jp}
• 关键词：Tissue-engineered muscle ; C2C12 ; Muscle contractility ; Myogenic regulatory factors ; Myogenesis
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：123
• 期：2
• 页码：265-271
• 全文大小：1973 K
• 卷排序：123

文摘

Tissue-engineered skeletal muscles were potentially useful as physiological and biochemical in vitro models. Currently, most of the similar models were constructed without tendons. In this study, we aimed to develop a simple, highly versatile tissue-engineered muscle with artificial tendons, and to evaluate the contractile, histological and molecular dynamics during differentiation. C2C12 cells were embedded in a cold type-І collagen gel and placed between two artificial tendons on a silicone sheet. The construct shrank and tightly attached to the artificial tendons with differentiation, finally detaching from the silicone sheet within 1 week of culture onset. We successfully developed a tissue-engineered skeletal muscle with two artificial tendons from C2C12 myoblasts embedded in type-І collagen gel. The isometric twitch contractile force (TCF) significantly increased during differentiation. Time to Peak Tension (TPT) and Half-Relaxation Time (1/2RT) were significantly shortened during differentiation. Myogenic regulatory factors were maximally expressed at 2 weeks, and subsequently decreased at 3 weeks of culture. Histological analysis indicated that myotube formation increased markedly from 2 weeks and well-ordered sarcomere structures were observed on the surface of the 3D engineered muscle at 3 weeks of culture. These results suggested that robust muscle structure occurred by 3 weeks in the tissue-engineered skeletal muscle. Moreover, during the developmental process, the artificial tendons might contribute to well-ordered sarcomere formation. Our results indicated that this simple culture system could be used to evaluate the effects of various pharmacological and mechanical cues on muscle contractility in a variety of research areas.