Investigating the structural transitions of proteins during dissolution by mass spectrometry

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• 作者：Xiaoyun Gong ; Xingchuang Xiong ; Lin Qi ; Xiang Fang
• 关键词：Structural transition ; Protein ; Dissolution ; Mass spectrometry
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：164
• 期：Complete
• 页码：418-426
• 全文大小：957 K
• 卷排序：164

文摘

Interactions between proteins and solvents are important for proteins to maintain their active structures. The structural transition of proteins during their dissolution process in different solvents was investigated. Solvent assisted electric field induced desorption/ionization (SAEFIDI) was used for the investigation. NH4Ac buffers caused significant unfolding of cytochrome c and myoglobin during their dissolution process. Different proteins underwent varied folding and unfolding transitions during dissolution.