Identification of human bocaviruses in the cerebrospinal fluid of children hospitalized with encephalitis in China

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• 作者：Jie-mei Yu^a ; ¹ ; Qian-qian Chen^b ; ¹ ; Ye-xia Hao^a ; Tian Yu^b ; Sai-zhen Zeng^b ; Xiao-bing Wu^a ; Bing Zhang^b ; ^{zhangbing1959415@yahoo.com.cn} ; Zhao-jun Duan^a ; ^{zhaojund@126.com}
• 关键词：HBoV ; human bocavirus ; CSF ; cerebrospinal fluid ; Ig ; immunoglobulin ; ELISA ; enzyme-linked immunosorbent assay ; IFA ; indirect immunofluorescence assay ; FITC ; fluorescein isothiocyanate
• 刊名：Journal of Clinical Virology
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：57
• 期：4
• 页码：374-377
• 全文大小：599 K

文摘

Background

Encephalitis is a major cause of death and disability in adults and children; different members in the family Parvoviridae are known to be associated with encephalitis to some extent.

Objectives

To determine the presence of human bocaviruses (HBoVs) and corresponding HBoV-specific immunoglobulins (Igs) in cerebrospinal fluid from children with suspected viral encephalitis.

Study design

Employing real-time PCR and nested touchdown PCR, 67 cerebrospinal fluid (CSF) samples from children with suspected viral encephalitis were screened for HBoV and routine encephalitis-causing viruses. Using ELISA, Western blot and IFA, HBoV-specific Ig were determined in the samples.

Results

Nine samples (134 % ) were HBoV1 DNA-positive, while one sample (15 % ) was HBoV2 DNA-positive. HBoV-specific IgM and IgG antibodies were detected in the CSF samples from three children; two samples were HBoV1 DNA-positive and one sample was negative. One death was recorded; CSF from this child was the only HBoV-IgM-positive CSF samples among the 67 CSF tested.

Conclusion

We screened CSF samples obtained from children with encephalitis for the presence of HBoV1 and HBoV2 and specific IgM- or IgG-responses. Detection of viral DNA and/or immunological response to HBoV1/HBoV2 highlights the significance of these viruses as causes of encephalitis in children. Further studies are needed to examine the role of HBoV infection in children encephalitis.