1D NMR methods for determination of degree of cross-linking and BDDE substitution positions in HA hydrogels

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• 作者：Frida J. Wende^a ; ^{Frida.Wende@slu.se} ; Suresh Gohil^a ; ^{Suresh.Gohil@slu.se} ; Lars I. Nord^b ; ^{Lars.Nord@galderma.com} ; Anne Helander Kenne^b ; ^{Anne.HelanderKenne@galderma.com} ; Corine Sandströ ; m^a ; ^{Corine.sandstrom@slu.se}
• 关键词：HA ; hyaluronic acid/hyaluronan ; MoD ; degree of modification ; CrR ; effective crosslinker ratio ; BDDE ; 1 ; 4-butanediol diglycidyl ether ; BDPE ; 1 ; 4-butanediol di-(propan-2 ; 3-diolyl)etherch ; ABC ; chondroitinase ABC ; GlcNAc ; N-acetyl-d-glucoseamine ; ΔGlcA ; d-glucuronic acid with C4-C5 unsaturation ; NOE ; nuclear overhauser effect ; SEC ; size exclusion chromatography ; TDA ; triple detector array
• 刊名：Carbohydrate Polymers
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：157
• 期：Complete
• 页码：1525-1530
• 全文大小：1306 K
• 卷排序：157

文摘

1D carbon NMR method to determine the degree of cross-linking in HA hydrogels was developed. 1D proton NMR method to determine the cross-linker position in HA hydrogels was developed. No chromatographic separation step is required prior to NMR analysis.