Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study

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• 作者：Yuting Cong^a ; ^b ; Lianghai Hu^a ; ^{lianghaihu@jlu.edu.cn} ; Zhang Zhang^b ; Yin Gao^a ; Mingming Dong^b ; Hongqiang Qin^b ; Mingliang Ye^b ; ^{mingliang@dicp.ac.cn} ; Jingkai Gu^a ; Hanfa Zou^b ; ¹
• 关键词：Proteomics ; Mass spectrometry ; Therapeutic monoclonal antibody ; Glycosylation ; Parallel reaction monitoring ; Pharmacokinetics
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 April 2017
• 年：2017
• 卷：165
• 期：Complete
• 页码：664-670
• 全文大小：742 K
• 卷排序：165

文摘

PRM enabled the qualitative and quantitative analysis of mAbs and its glycosylation. Absolute quantitation of mAbs and their glycoforms was achieved in serum and tissues. HILIC enrichment enabled the in-depth analysis of low abundant glycopeptides.