One-step separation-free detection of carcinoembryonic antigen in whole serum: Combination of two-photon excitation fluorescence and optical trapping

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• 作者：Cheng-Yu Li ; Di Cao ; Chu-Bo Qi ; Hong-Lei Chen ; Ya-Tao Wan ; Yi Lin ; Zhi-Ling Zhang ; Dai-Wen Pang ; Hong-Wu Tang ; ^{hwtang@whu.edu.cn}
• 关键词：Optical trapping ; Two-photon excitation ; Carcinoembryonic antigen ; Separation-free
• 刊名：Biosensors and Bioelectronics
• 出版年：2017
• 出版时间：15 April 2017
• 年：2017
• 卷：90
• 期：Complete
• 页码：146-152
• 全文大小：593 K
• 卷排序：90

文摘

An analytical setup based on NIR optical trapping and two-photon fluorescence was constructed. Quantitative detection was realized by trapping single beads with fluorescence imaging. One-step separation-free detection of biomarker CEA in human whole serum was conducted. The assay for real samples is concordant with the results by standard chemiluminescence method.