Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycol

详细信息    查看全文

• 作者：Pavel Marichal-Gallardo^a ; ¹ ; ^{marichal-gallardo@mpi-magdeburg.mpg.de} ; Michael M. Pieler^a ; ¹ ; Michael W. Wolff^a ; ^b ; Udo Reichl^a ; ^c
• 关键词：aHA ; hemagglutination activity ; BSA ; bovine serum albumin ; ciVB ; clarified inactivated virus broth ; CMA ; cellulose membrane adsorber ; CV ; column volume ; DCS ; differential centrifugal sedimentation ; dsDNA ; double stranded DNA ; DSP ; downstream processing ; DBC5 ; dynamic binding capacity at 5% breakthrough ; EBC ; equilibrium binding capacity ; FCS ; fetal calf serum ; FT ; flow-through ; HA ; hemagglutinin ; HAU ; hemagglutination units ; HIC ; hydrophobic interaction chromatography ; hpi ; hours post infection
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：3 February 2017
• 年：2017
• 卷：1483
• 期：Complete
• 页码：110-119
• 全文大小：1071 K
• 卷排序：1483

文摘

Cellulose membranes are a suitable matrix for steric exclusion chromatography. Virus particles were eluted in Tris-HCl and PBS buffers with >95% recovery. Maximum depletion of host cell DNA and protein was 99.7% and 92.4%, respectively. Operation is single-use and fully scalable to industrial manufacture. The DBC5 of the hemagglutinin antigen was 3.4 μgHA cm−2 (906 μgHA mL−1).