Off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry to improve coverage in lipidomics analysis

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• 作者：Mó ; nica Narvá ; ez-Rivas^a ; Ngoc Vu^b ; Guan-Yuan Chen^a ; Qibin Zhang^a ; ^b ; ^{q_zhang2@uncg.edu}
• 关键词：Rat plasma lipidome ; Rat liver lipidome ; Off-line two-dimensional liquid chromatography ; Untargeted lipidomics ; Monolithic column ; Accucore C30 column
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：954
• 期：Complete
• 页码：140-150
• 全文大小：1196 K
• 卷排序：954

文摘

Mixed mode LC fractionation coupled with reversed phase LC-MS/MS for lipidomics. 22 lipid classes were separated in mixed mode on a monolithic Si-based column. Reduced ion suppression and cleaner spectra obtained from fractionated samples. Total fatty acid analysis to improve confidence of identified lipid in LipidSearch. More than 800 lipids were confidentially identified in rat plasma and rat liver.