Semaphorin 4D Enhances Angiogenic Potential and Suppresses Osteo-/Odontogenic Differentiation of Human Dental Pulp Stem Cells

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• 作者：Ting Zou ; MS^&lowast ; ; Waruna Lakmal Dissanayaka ; BDS ; PhD^&lowast ; ; Shan Jiang ; DDS ; MS^&dagger ; ; Shuai Wang ; BDS ; MS^&lowast ; ; Boon Chin Heng ; BS ; PhD^&lowast ; ; Xiaojing Huang ; DDS ; PhD^&dagger ; ; Chengfei Zhang ; DDS ; PhD^&lowast ; ; ^{zhangcf@hku.hk}
• 关键词：Alkaline phosphatase activity ; angiogenic potential ; dental pulp stem cells ; osteo-/odontogenic differentiation ; semaphorin 4D/plexin-B1
• 刊名：Journal of Endodontics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：43
• 期：2
• 页码：297-305
• 全文大小：1516 K
• 卷排序：43

文摘

Semaphorin 4D (Sema4D)-treated dental pulp stem cells displayed marked down-regulation in the expression of osteo-/odontogenic genes (ALP, Col1A1, BSP, RUNX2, and DSPP) as well as proteins (Col1A1 and DSPP). Sema4D-treated dental pulp stem cells exhibited enhanced expression of vascular endothelial growth factor at both the messenger RNA and protein level. Sema4D markedly enhances the angiogenic potential but suppresses osteo-/odontogenic differentiation of dental pulp stem cells via the RhoA-mediated pathway. Elevated levels of plexin-B1 and downstream RhoA protein expression together with phosphorylated plexin-B1 confirmed the involvement of Sema4D/plexin-B1 signaling.