Immunoassays of chemically modified polysaccharides, glycans in glycoproteins and ribose in nucleic acids

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• 作者：Slađana Strmečki^a ; ¹ ; Mojmí ; r Trefulka^a ; Pavlí ; na Zatloukalová ; ^b ; Michal Durech^b ; Borivoj Vojtesek^b ; Emil Paleček^a ; ^b ; ^{palecek@ibp.cz}
• 关键词：Carbohydrate chemical modification ; Os(VI) complexes ; Antibody against chemically modified carbohydrates ; Glycoprotein adduct immunoassays ; Voltammetry of chemically modified glycoproteins ; Carbon and mercury electrodes
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：22 February 2017
• 年：2017
• 卷：955
• 期：Complete
• 页码：108-115
• 全文大小：1503 K
• 卷排序：955

文摘

A new monoclonal antibody Manost 2.1 was raised against mannan-Os(VI)temed. Manost 2.1 specifically recognized Os(VI)temed-modified glycans. Mixtures of chemically modified glycoproteins were analyzed by Western blotting. Sensitivities of dot blot immunoassay and electrochemical analysis were comparable.