Highly sensitive detection of multiple tumor markers for lung cancer using gold nanoparticle probes and microarrays

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• 作者：Wanlei Gao^a ; ^e ; ¹ ; Wentao Wang^a ; ^b ; ¹ ; Shihua Yao^c ; Shan Wu^a ; ^d ; Honglian Zhang^a ; Jishen Zhang^a ; Fengxiang Jing^a ; Hongju Mao^a ; Qinghui Jin^a ; Hui Cong^d ; Chunping Jia^a ; ^{jiachp@mail.sim.ac.cn} ; Guojun Zhang^b ; ^{zlgj-001@126.com} ; Jianlong Zhao^a
• 关键词：Microarray ; Gold nanoparticles ; Gold deposition ; Tumor marker ; Lung cancer
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：15 March 2017
• 年：2017
• 卷：958
• 期：Complete
• 页码：77-84
• 全文大小：1828 K
• 卷排序：958

文摘

A co-detection method based on gold nanoparticles and microarrays is reported for detecting multiple biomarkers. A gold nanoparticle/antibody composite is synthesized as detecting probe. The gold deposition staining with HAuCl4 and H2O2 produces a color change that can be observed with the naked eye. This protein biochip is capable of detecting multiple biomarkers in 12 serum samples simultaneously in 1 h. The results confirm the clinical significance of DKK1 as a serologic marker of lung cancer.