Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen

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• 作者：Kun Xiao^a ; ^{kun_x@foxmail.com} ; Kan Wang^a ; ^b ; ^{wk_xa@163.com} ; Weijian Qin^a ; ^{1602701580@qq.com} ; Yafei Hou^a ; ^{yafeihou@sjtu.edu.cn} ; Wenting Lu^c ; ^{luwenting23@163.com} ; Hao Xu^d ; ^{xudahao75@qq.com} ; Yan Wo^e ; ^{woyansh@163.com} ; Daxiang Cui^a ; ^b ; ^{dxcui@sjtu.edu.cn}
• 关键词：ICTS ; immunochromatographic test strip ; CEA ; carcinoembryonic antigen ; NSE ; neuron specific enolase ; CA72-4 ; carbohydrate antigen 72-4 ; PSA ; prostate specific antigen ; AFP ; alpha-fetoprotein ; QBs ; quantum dot beads ; QDs ; quantum dots ; SCLC ; small cell lung cancer ; EDC ; 1-ethyl-3-(3- dimethyllaminopropyl)-carbodiimide hydrochloride ; BSA ; bovine serum albumin ; NHS ; N-hydroxy-succinimide ; PBS ; phosphate buffer saline ; PVC ; polyvinyl chloride ; NC ; nitrocellulose ; T1 ; test line 1 ; T2 ; test line 2 ; C
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：164
• 期：Complete
• 页码：463-469
• 全文大小：950 K
• 卷排序：164

文摘

The good monodispersity and high colloidal stability quantum dot nanobeads were applied as label materials to the test strip. Utilization of fluorescent test strip to simultaneously detect neuron specific enolase and carcinoembryonic antigen. A novel optimal reader system “Handing” had been employed for highly sensitive multiplex assay of test strips.