Electrochemical immunosensor for N6-methyladenosine detection in human cell lines based on biotin-streptavidin system and silver-SiO₂ signal amplification

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• 作者：Huanshun Yin ; Haiyan Wang ; Wenjing Jiang ; Yunlei Zhou ; ^{zhyl@sdau.edu.cn} ; Shiyun Ai ; ^{ashy@sdau.edu.cn}
• 关键词：RNA methylation ; N6-methyladenosine ; Electrochemical immunosensor ; Phos-tag-biotin ; Ag@SiO2 nanocomposite
• 刊名：Biosensors and Bioelectronics
• 出版年：2017
• 出版时间：15 April 2017
• 年：2017
• 卷：90
• 期：Complete
• 页码：494-500
• 全文大小：950 K
• 卷排序：90

文摘

An electrochemical immunosensor was fabricated for N6-methyladenosine detection. Ag@SiO2 was prepared and used as signal amplification label. Phos-tag-biotin played a vital role of “bridge” to link m6ATP and Ag@SiO2. The developed method was validated for detect m6A content in human cell lines.